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Despite encouraging preclinical findings, clinical data are currently absent, and prioritization of early-phase human trials is necessary before any definitive conclusions can be drawn

Cell pellet was lysed in 40 L RIPA buffer (Thermo Fisher Scientific 89901) containing protease inhibitor cocktail (Sigma-Aldrich 11697498001) and incubated on ice for 10 min

Kavvadas, P

Hearts were minced finely in isolation buffer (300 mM sucrose, 10 mM Hepes, 2 mM EGTA, pH 7.2) at 4C, in the presence of 0.5 M type I protease (bovine pancreas) to release mitochondria from the muscle fibers, and then washed in the same buffer in the presence of 1 mg/mL BSA