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Moreover, you need to ensure that the amount of antibody used is enough to coat the entire bead or youll increase the risk of getting elevated background signal due to the nonspecific binding of the lysate components to the unsaturated portions of the beads

& McCullough, A

Similarly, all of the ApGST sequences observed in the EVs and EV surface, and 7 of the 9 ApGST sequences in EV-depleted ESP were also classified, or homologous to, Mu class GST representatives