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The transfected cells were cultured for a further 24 hours then lysed with RIPA buffer and prepared for gel electrophoresis

Obstacles and limitations In spite of promising in vitro and in vivo results, certain hurdles need to be overcome before resveratrol can be widely considered in humans

1981 May;34(5):971-2

43,47,48 Trials lasting up to seven days typically used doses of 20 grams per day and longer trials (224 weeks) used 5 grams per day, but optimal doses for raising brain creatine concentrations and enhancing brain function are not yet known