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Cells on day 21 from both differentiation methods demonstrated characteristic nuclear staining of HNF4A, and cytoplasm expression of AFP, ALB, CK18, A1AT and CYP3A4 (Fig

PTMs are critical molecular events in a series of biological processes such as cell growth, proliferation, differentiation, metabolism, and apoptosis ( Protein oxidation Cysteine residues in proteins are easily oxidized by ROS, reactive nitrogen species, reactive sulfur species, or GSH

Oxidative stress arises from an imbalance between reactive oxygen species (ROS) and the livers antioxidant defenses, contributing to cellular injury and chronic inflammation

Proceedings of the National Academy of Sciences, 87: 45334537