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In this compartment GSH might get oxidized, especially, when direct GSH import is abolished, and the resulting GSSG could then be transported by Gsi

Oxidized peroxiredoxin-2 has a significantly reduced capacity to neutralize hydrogen peroxide, which is then decomposed by traces of redox-active iron to hydroxyl radicals

Enzyme samples were incubated with phosphate buffer, which included the appropriate concentrations of glutathione (GSH) and peroxide as substrates, to determine Gpx activity