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It has been observed that the conversion from 2-hydroxysetrogens to 4-hydroxyestrogen might be an additional pro-inflammatory signal because these endogenous metabolites can be oxidized to 3,4-quinone, which lead to depurination and mutation [49]

Heres the whole thing in plain terms: What they did: built and validated a lab-grade method to accurately measure 5A1MQ in the blood, then dosed rats two ways intravenous (IV) and oral and tracked exactly how much showed up in circulation over time

SEC analysis allows both for sizing of aggregates, and their quantification

These harmful variations disrupt the function of the glucocerebrosidase enzyme, leading to reduced enzymatic activity and potentially increasing the aggregation of -Syn (Sidransky et al