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Molecular and cellular outcomes were assessed most frequently using Western blotting (n = 10) to identify protein expression, or through various polymerase chain reaction (PCR) techniques (n = 8) to quantify gene expression in the cells

[DOI] [PubMed] [Google Scholar] [33].Lerman LS, A biochemically specific method for enzyme isolation, Proc

doi:10.1056/NEJMoa2301972 PubMed: This landmark study demonstrated 24.2% mean weight loss at 48 weeks with the 12mg dose in 338 adults with obesity, establishing retatrutide as the most effective obesity medication tested to date