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The addition of exogenous ROS results in resensitization of cells with high VRK2 expression to GSH inhibition, which is consistent with the fact that PC cells with low expression of VRK2 are susceptible to GSH inhibition attributes to its high basal ROS level, suggesting that cells in a state of oxidative stress have enhanced dependence on the GSH system

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Annexin V-PI apoptosis assay After completing each group treatment, the culture medium in the plate was collected and transferred to a 15 mL centrifuge tube

In summary, MOTS-c powerfully supports muscle metabolism by improving insulin sensitivity, boosting glucose and fatty acid utilization, and even mimicking some effects of endurance exercise a profile that is highly promising for addressing metabolic health and age-related muscle decline