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ROS levels in the cells were detected using an ROS Assay Kit (#S0033, Beyotime) based on the DCFH-DA method

Those most at risk include vegans, seniors, individuals with gastrointestinal absorption issues, and anyone who has undergone weight-loss surgery

As FDA states in their guidance document on the Q-Submission program, Early interaction with FDA on planned non-clinical and clinical studies and careful consideration of FDAs feedback may improve the quality of subsequent submissions, shorten total review times, and facilitate the development process for new devices. While this is not meant to be a general pre-review of your submission, it is there to help you get answers to questions directly from FDAwhich will contribute to a smooth submission and, hopefully, approval