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Therapeutic Research Protocol Dose: 300 600 mcg (1 2 capsules) Duration: 6 10 weeks Frequency: Once daily, preferably before physical-activity windows Cycle Interval: 3 4 weeks off before repeating Goal / Description: Used in research targeting endurance, fat oxidation, and ERR pathway activation

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Flow cytometry Cells grown on tissue culture plates were rinsed three times with KRH buffer [12 mM Hepes (pH 7.0), 121 mM NaCl, 4.9 mM KCl, 1.2 mM MgSO 4 , and 0.33 mM CaCl 2 ] and then blocked with 5% fetal bovine serum (FBS) in KRH buffer at 4C