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PCR was conducted in a thermal cycler with the following parameters: initial denaturation was at 95C for 5 min and was followed by 40 cycles of denaturation at 95C for 10 s and annealing and extension at 60C for 30 s

PBMCs were resuspended and seeded into 6-well plates at a density of 1 10 6 cells/cm 2 for adhesion-dependent monocyte enrichment

Oxidative StressInduced Cancer Oxidative stress usually causes damage to nucleic acids, proteins, and lipids, which are associated with heightened risk of tumorigenesis [142]

Distinguishing direct pharmacological anti-inflammatory actions from secondary metabolic improvements requires further mechanistic studies and randomized controlled trials specifically designed to assess inflammatory outcomes as primary endpoints