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Description

2.7 Immunocytochemistry: preparation of Alexa Fluor-labeled proteins, cell surface binding, endocytosis, and downregulation of TfRs HemeHPX in PBS was fluorescently labeled using Alexa Fluor (AF) 546 or 647, according to the manufacturers instructions (Invitrogen)

Endocannabinoid control of food intake and energy balance

Fully hydrolyzed BP tetrol (BPT) was washed from the column with 80% acetonitrile (at ~ 50 min)

To exclude the possibility of extracellular interactions between EPS and MPP + in vitro, we performed LCMS/MS analysis on the mixture of EPS and MPP +