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Because of the relatively low cysteine and methionine amount in leaves, the modest sensitivity of 33 S-NMR analyses and also the fact that sulfur assimilation represents a small metabolic flux (about 0.01 mol m 2 s 1 , that is, between 0.05 and 0.1% of photosynthesis), analyses required a considerable acquisition time, representing a total of nearly 3000 h for isotopic measurements

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