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In its simplest format, individual GSTs are expressed in microbial, or plant hosts, and then subjected to metabolic profiling to identify intermediates that accumulate due to binding interactions with the ligandin. Using this methodology with tau class proteins expressed in Escherichia coli , we identified that maize ( Zea mays ) Zm GSTU1 and Zm GSTU2 interacted with porphyrinogen intermediates and caused the hyperaccumulation of colored porphyrins ( At GSTUs were expressed in E

DOI: 10.1038/s41586-020-2286-9 Ziegler C et al

Tikhonravov D, Neuvonen T et al (2008)