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[45] However, no sufficient evidence has been found to explain the indispensability of seawater for obligate marine yeasts

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Bacterial DNA was isolated from the feces using a QIAamp PowerFecal DNA Kit (12830-50, QIAGEN), and PCR was carried out with 50 g DNA template to quantify 16S DNA gene expression using bacterial primers (8F and 338R) and V6

Instead, they are recommending that if the clinicians find from radiographs that a dog's vertibral heart score (VHS) value is at least 11.5, it would be perfectly proper to skip the echocardiogram and administer pimobendan to that dog, regardless of whether the dog's heart is actually enlarged or not