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Taking a good quality vitamin D3 supplement on a regular basis and testing your 25-OH vitamin D3 levels a few times a year to make sure it is in the optimal 50-100 ng/mL range is recommended

Proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon FL 0,45 m, Merck) by semi-dry electroblotting (0.8 mA/cm 2 , 1 h) using a Transblot SD apparatus (Bio-Rad)