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Fms-Like Tyrosine Kinase 3 Ligand (Flt3L)-Differentiated Dendritic Cell (FL-DC) Treatment FL-DCs were prepared as previously described ( 5 cells/ml in RPMI-1640 medium containing 10% FBS supplemented with 200 ng/ml recombinant murine Flt3L (rmFlt3L, PeproTech, Rocky Hill, NJ, USA) and 2-mercaptoethanol (50 M, Sigma-Aldrich), for 8 d