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The FASEB Journal , 29

Do not freeze it, as the freeze-thaw cycle can degrade the peptide

Additionally, in a more recent meta-analysis (Wei et al., 2022), conducted from 14 studies covering data from 623 dairy cows, and considering the content of metabolizable amino acids that eventually reach the small intestine from different sources of RPM, it was found that adding RPM to the diet did not significantly improve the milk yield of the dairy cows

The correct technique: insert the needle, allow the vacuum in the lyophilised vial to draw the solvent in passively, then withdraw the needle without injecting air