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HuR biological function involves RRM3-mediated dimerization and RNA binding by all three RRMs

In contrast, cancer cells loose this regulated homeostasis in several ways, including alterations in intrinsic and extrinsic molecular mechanisms that govern cellular metabolism, in order to provide the basic metabolic requirements of tumoral cells, such as quick biosynthesis of ATP, accelerated biosynthesis of macromolecules, and maintenance of optimal redox status (Cairns et al., 2011)

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Polymerization occurred at 37 C for 30 min, culture medium was overlaid and image capture performed as previously described (protocols.io For Boyden chamber invasion assays, cells were incubated in 0.1% inactivated BSA for 24 h and then plated in Matrigel-covered Boyden chambers (6 g/96-well, bottom treated with collagen type I) and incubated for 8 h at 37 C and 5% CO 2 atmosphere, with 5 ng/mL of epithelial growth factor as a chemoattractant