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First, we sought to identify a medium that would support assembloid formation, that is, the culture of all three cell types: hepatocytes, cholangiocytes/ductal cells and portal mesenchyme without overgrowth of any of them, we tested several media and found that a minor adaptation of the DM medium used for h-HepOrgs differentiation without A8301 (assembloid medium) supported culture of the three cell types while preventing their overgrowth

Although the precise mechanisms that underlie the observed differences between the neutralizing titers elicited by membrane-anchored gH/gL and the ectodomain are not clear, we note that a similar phenomenon was observed when comparing mRNA delivery of membrane-anchored and secreted MERS Spike protein 63 , and that mRNA-based SARS-CoV-2 vaccines are based on full length rather than secreted spike proteins 63,64,65,66

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