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Drug Development Res 1988;14:213-6

Immunofluorescence and confocal microscopy Cells were washed with PBS, fixed for 20 min in 4% (w/v) PFA, permeabilised in PBS/0.1% (v/v) Triton X-100 (PBS-T), blocked with PBS-T/5% (w/v) BSA before staining with primary antibody (sheep anti-NS5A 1:2000, mouse anti-NS5A

L.MurphyK

While these methods may help with the stress related to thyroid disease, there is no strong evidence showing that they effectively treat thyroid disorders