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At each time point, 20 L of iHLO culture medium was transferred to a 384-well white flat-bottom plate, 20 L of Luciferin Detection Reagent was added, and the mixture was incubated for 30 min before measuring luminescence

Symptoms include: Persistent fatigue and lack of motivation Decreased libido or erectile dysfunction Loss of muscle mass and strength Weight gain and slower metabolism Mood changes, irritability, and poor focus Reduced bone density A meta-analysis published in Clinical Endocrinology confirmed that TRT significantly increases lean body mass and decreases fat mass in hypogonadal men (Corona et al., 2016)

Nakamura, T

Neurology 102 (7 Supplement 1), 3472