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In brief, an aliquot (200 mg) of each sample was suspended, while frozen, in a solution containing 500 l of freshly prepared extraction buffer (200 mM Tris, 200 mM NaCl, 20 mM EDTA, pH of 8.0), 200 l of 20% SDS and 500 l of a mixture of phenol:chloroform:isoamyl alcohol (25:24:1) in 2 ml VK05 Precellys tubes containing 0.5 mm glass beads (Bertin Technologies)

Funding EKP is a recipient of the major research project (MJRP) grant (SIU/SCRI/MJRP- Approval/2024/1397) from Symbiosis International (Deemed University)

* Washout period refers to the time from the last administered dose to the time of the start of the In-Competition period (i.e

While many individuals can benefit from B12 injections, certain groups may see more significant or rapid improvements: Vegetarians and Vegans Plant-based diets are often low in B12, making supplementation crucial