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All animals were housed at Animal Experiment Center of Wenzhou Medical University under the following conditions: 3 mice/ cage (30 20 15 cm, length width height), 12 h dark/light cycle, room temperature (24 1) C, ambient humidity (50 10) %, with free access to food and water

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However, the toxicity of the mediators required for high efficiency is a major bottleneck

Before each extraction, 5 L were taken from every CSF sample and pooled together separately in a 2 mL centrifuge tube to create a pooled quality control (QC) sample used for normalisation and for calibration