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Description

The reduced ingredient should lead the formula

Kourie JI

They worked in pairs, and disagreements were resolved by the fifth researcher (RC)

Reactions were performed in 50 mM sodium phosphate buffer pH 7.0 containing 0.1% Triton-X-100, 0.40 mM EDTA, 0.20 mM NADPH, 0.25 U glutathione reductase from Bakers yeast, 1 mM L-glutathione, and 100 M t -butyl hydroperoxide (all Sigma-Aldrich) in a total volume of 200 l