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Because the concentration is the same as the 10 mg with 2 mL example, a 2.5 mg dose is still 0.5 mL, or 50 U-100 units

Cells were used within three months post-thawing and cultured in RPMI 1640 medium (#01859-34, Nacalai Tesque, Kyoto, Japan) with 10% fetal bovine serum (#30264-85, Nacalai Tesque, Kyoto, Japan), 2 mM glutamine, 50 U/mL penicillin, and 100 g/mL streptomycin (#26253-84, Nacalai Tesque, Kyoto, Japan)

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First, since chained inhibitory neurons shape the output signals through the mechanism of disinhibition/inhibition, impairments of GABA release and LTD distort the conversion process from the internal model to the output signals