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massiliensis were then extracted from quality-filtered reads by using blastn algorithm [38] ( e value 50% stenosis for at least one coronary vessel) were recruited as an independent cohort for validating the microbiome-based TMAO producer prediction model (the baseline characteristics of CVD validation cohort were described in Table S2)

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Among non-invasive imaging modalities, abdominal ultrasonography remains the most commonly utilized due to its accessibility and cost-effectiveness, although its sensitivity decreases in patients with lower degrees of steatosis

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