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According to data from the literature, significant events in the formation of a stroke area, such as excitotoxicity, mitochondrial damage, emergence of reactive oxygen species, and apoptosis, occur within the first 3 h after occlusion of an artery [53], and the expression of genes at the early stage of ischemia can be studied at this time point

Critics note that much of this research comes from Russian institutions with limited peer review in Western journals

After reconstitution for laboratory use, keep cold and use promptly

Before adding 25l of Universal Assay Buffer (UAB) to each well, beads were washed twice using a hand-held magnetic plate washer