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The cells were washed twice in 1 mL ice-cold PBS and lysed with 5 pellet volume of RIPA buffer (20 mM HEPES, 100 mM NaCl, 0.1% Triton-X, 0.2% SDS, PhosStop, cOmplete inhibitor tablet)

Moreover, it was shown experimentally that Gch1 (gene encoding the rate-limiting enzyme of BH4 biosynthesis) is a direct target of Nrf-2 in skin cells submitted to radiation (Nrf-2 has a binding site in the proximal promoter of the Gch1 gene), and the resultant increased intracellular BH4 levels promoted cytoprotection by neutralizing ROS [108]

A timer was started once the experimenter released the mouse to allow it to hang freely

Through this, the patients health history, diet, and other related details may be gathered and factored in