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For quantitative reverse transcription PCR (RTqPCR), 1,000 ng of total RNA was reverse transcribed into complementary DNA (cDNA) using a Hifair II 1st Strand cDNA Synthesis Kit (Yeasen, China) following the manufacturers instructions

Activation of HIF1 rescues the hypoxic response and reverses metabolic dysfunction in the diabetic heart

Autoimmune mechanisms and inflammation in obesity-associated type 2 diabetes, atherosclerosis, and non-alcoholic fatty liver disease

These cells produce soluble molecules, including members of the tumor necrosis factor superfamily (TNFSF), which diffuse through the tissue and bind to their receptors on target cells, leading to the progression of apoptosis (Yanumula and Cusick, 2023)