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FeS clusters Ironsulfur clusters are essential enzyme cofactors

Measure of competitive binding titers by ELISA Coating, blocking, and gH/gL immobilization steps were performed as described under Measurement of serum antibody endpoint binding titers by anti-His capture ELISA. Following the capture of monomeric gH/gL, equal amounts of sera from each mouse in a group were pooled and diluted in blocking buffer and 2-fold serial dilutions were performed, followed by a 1 h incubation at 37 C

Results depend on consistency and individual response

If you cant do that, then eventually your thyroid gland will start to damage itself which may result in immune cell infiltration and eventually permanent thyroid gland destruction