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Mice were placed in a stereotaxic frame (Kopf Instruments, USA), and a rectangular craniotomy was performed over the medial prefrontal cortex (AP, 13 mm and ML, 01.5 mm from the bregma) using a dental drill (Microtorque II, Ram Products, USA)

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In terms of safety, TRT has its own considerations (polycythemia, prostate effects, etc.) which are unrelated to PT-141s profile