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[15] In hepatocytes, if all glucose currently stored as glycogen were instead present in its free form, the intracellular glucose concentration would rise to about 0.4 M, compared to the actual polysaccharide concentration of roughly 0.04 M

276 Additionally, functional and structural changes have been observed at the level of band 3

The medium was half replaced every other day and cells were passaged with Accutase (Sigma) every 7 days and used for subsequent experiments after 24 passages

These evidences recommend further investigation into whether and how neuromelanin and -synuclein directly interact to drive neurotoxicity and neurodegeneration in PD