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These reactions introduce or unmask functional groups (e.g., hydroxyl, carboxyl, amino) on the molecule, often resulting in the formation of intermediate metabolites

The most often and potent ones attracted are the insulin receptor substrates 1/2 (IRS1/2)although there are 6 found to date- (not to forget that Shc proteins, p60dok, Cbl, APS, and Gab-1 are also recruited to activated IRs)

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When the ligand aligned to create a favourable interaction, LibDock used features of protein polar and apolar interaction sites, known as hot spots