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This functional response, which also enhances the phagocytosis of apoptotic cells and collagen deposition, serves to protect the host from inflammatory damage and promote tissue healing ( During the initial phase of mycobacterium tuberculosis infection, the expression of IFN- and iNOS in the BALF of mice gradually increased, aligning with the observed trend of AM polarization, indicating M1-biased AM polarization

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Methods of improving the ability of a genetic vaccine vector to modulate an immune response Also provided by the invention are methods of improving the ability of a genetic vaccine vector to modulate an immune response by: (1) reassembling (&/or subjecting to one or more directed evolution methods described herein) at least first and second forms of a nucleic acid which comprise a genetic vaccine vector, wherein the first and second forms differ from each other in two or more nucleotides, to produce a library of recombinant genetic vaccine vectors