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dilution as indicated by the manufacturer) was performed following xylene deparaffinization, rehydration, heatinduced epitope retrieval with 10 mM sodium citrate buffer (0.05% Tween, pH 6.0), quenching of endogenous peroxidase activity by hydrogen peroxide incubation, and blocking in 5% normal goat serum in PBS

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However, the studys lack of a control group, small sample size, short follow-up, and the use of multiple simultaneous therapies limit the ability to attribute the observed benefits specifically to shock wave therapy.53 Thus, the SWT appears to be a promising therapeutic approach for lipedema, warranting further investigation through controlled trials