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As a result, the imbalance in MET can intensify glycolysis, leading to substantial lactate generation, which, in turn, weakens CTL activity and promotes immune evasion by tumors

doi: 10.1111/imr.13419 142 TavaresR

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If you must freeze, aliquot the reconstituted solution into single-dose vials immediately after mixing so each dose undergoes only one freeze-thaw event