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24 h later, 20 l medium with compounds at 5X of the desired concentration was added to the well for 24 h

Yes, though its already comprehensive

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Sigma-Aldrich, Madrid, Spain) and cultured in RPMI 1640 medium supplemented with 10% heat-inactivated FBS, penicillin, streptomycin, L-glutamine, and 50 U/mL of human recombinant interleukin 2 (IL2) [49]