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The activity of the antioxidant enzyme GPx was determined by standard protocols, in blood samples (2 ml each), collected from all subjects, after the onset of arrhythmia (for groups I and II), by venipuncture (in K2EDTA vacutainers), using a standardized Ransel Laboratory kit (RandoxLaboratories, Ltd.)

Before the clinical study, a primary dermal irritation study was performed on 35 healthy volunteers (13 male and 22 female)

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Excessive ionophore administration can overwhelm these defenses, leading to systemic copper poisoning, characterized by hepatotoxicity, nephrotoxicity, and hematopoietic suppression [239]