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HC estimates are used as levels of concern protective to a broad array of aquatic species, as they inform of potential impacts and are used to derive PNECs because they are a community-level threshold making full use of the available data on the toxicity of a chemical to standard and non-standard toxicity test species and so relate concentration to ecological impacts (i.e., the proportion of species at risk)

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However, the number and size of metastases were much higher when B16M cells with high GSH content were inoculated in vivo [33]