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Following vortexing and passage through a 70 m strainer, the suspension of IECs was washed twice by centrifugation at 600 g for 3 min and collected for subsequent experiments

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AMA Drug Evaluations

Box 2 (shipped on blue ice packs) Material not included 96-well microtiter plate Distilled or deionized water 1X PBS 10 L to 1000 L adjustable single channel micropipettes with disposable tips 50 L to 300 L adjustable multichannel micropipette with disposable tips Conical tubes and bottles for sample and buffer preparation Centrifuge and/or microfuge Sonicator or tissue homogenizer Multichannel micropipette reservoirs Ethanol Microplate reader capable of reading 405 nm Application Notes Optimal working dilution should be determined by the investigator