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Two pieces of 12 mm round sterile glass coverslip attached to the lid of the 24-well plate were immersed into the suspension using the Amsterdam Active Attachment (AAA) model as previously described by Pakkulnan et al

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The drug concentration in the tumor reached the therapeutic threshold without harming adjacent normal cells

In summary, through pan-cancer analysis using the TIMER database combined with mechanistic integration, this review demonstrates that the activation patterns of the serine synthesis pathway (SSP) in malignant tumors are not randomly distributed, but follow three distinct molecular subtypes: driver mutation-dependent, microenvironmental stress-adaptive, and epigenetic dysregulation subtypes