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Confluent cell cultures were washed once with PBS, and then incubated for 20 min at room temperature with NucBlue Live ReadyProbes Reagent (#R37605, ThermoFisher Scientific, Waltham, MA, USA) to stain the nuclei, washed with PBS, permeabilized with 0.1% Triton-X 100 for 30 min, fixed at 37 C for 20 min with 4% Paraformaldehyde in PBS, and blocked with 2% BSA in PBS at room temperature for 20 min

Even though there are concerns for the development of kidney disease in CPTII patients due to the high metabolic overload associated to rhabdomyolysis, end-stage chronic renal insufficiency requiring dialysis is only occasionally reported in these patients (Joshi and Zierz, 2020)

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Child Psychiatry Hum Dev 42 , 367375 (2011)