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Comparison between the effects of torsemide and furosemide on the renin-angiotensin-aldosterone system of normal dogs

Martnez-Montas, F

The common denominators of all these processes include ROS-induced oxidative stress, dysregulation of antioxidant mechanisms, and inactivation of enzymes

Spheroid three-dimensional cultures were prepared using A431 cells seeded on multi-well plates precoated overnight at room temperature with 1.2% poly-HEMA (2-hydroxyethyl methacrylate, Sigma-Aldrich) to prevent cell adhesion, and maintained in a specific medium made of 1:1 DMEM:F12 (F-12 Nutrient mixture, Ham, Gibco, Thermo Fisher Scientific Inc., Rockford, IL, USA), 2% B27 serum free supplement (Gibco), 20 ng/ml of EGF (Sigma-Aldrich/Merck), 0.4% BSA and 4 g/ml of insulin (Gibco)