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Key Takeaways MBP is the most effective general-purpose solubility enhancer , improving soluble expression 23x more often than other tags in systematic comparisons SUMO offers the cleanest cleavage (native N-terminus after Ulp1/SENP removal) with meaningful solubility enhancement at a fraction of MBP's size GST dimerizes a feature or a problem depending on your downstream application Tag removal often causes precipitation : ~30% of proteins that are soluble as fusions aggregate after cleavage, so plan for this upfront The "right" fusion partner depends on your protein's specific problems : PTM requirements, disulfide bonds, size, and downstream application should drive the choice Why Fusion Partners Work The Problem They Solve Many recombinant proteins fail to fold properly in E

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doi: 10.1016/j.nbd.2011.12.031

The authors identified an autosomal recessive juvenile parkinsonism gene located on chromosome 6 as the parkin RING-finger ubiquitin ligase and multiple classes of deletion mutants in the gene