Drosophila studies showed 11-16% extension Telomerase activation: In vitro studies confirmed hTERT upregulation in human fetal fibroblasts and pulmonary cells, with treated cells exceeding the Hayflick limit by approximately 10 additional population doublings Telomere elongation: Direct measurements showed statistically significant telomere lengthening in treated cells Cancer incidence: Long-term animal studies showed reduced spontaneous tumor formation, partially addressing the telomerase-cancer concern 2025 validation: An independent study confirmed telomere-lengthening effects across multiple human cell lines, representing the first significant non-Khavinson replication Key limitations: The evidence base is dominated by a single research group
DPPOS Study Design The DPPOS follow-up study started in 2002
Goal: Maintain testicular volume and function during TRT Approach: HCG 500 IU subcutaneously every other day (3x weekly) Begin same time as testosterone therapy Continue for duration of TRT Monitoring: Baseline testosterone, estradiol, LH, FSH Recheck at 6 weeks, then quarterly Adjust based on estrogen response Expected results: Maintained testicular size Preserved fertility potential Possible slight testosterone boost above TRT alone Protocol for fertility restoration on TRT Men on TRT who want to conceive face a decision
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