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After incubation for 20 min at room temperature, the siRNA-lipid complex was then added gently into cells in a spiral pattern

doi: 10.1371/journal.ppat.1000494 39 ChongSZWongKLLinGYangCMWongSCAngeliVet al

Smoking-induced adipose dysfunction is characterized by altered adipocyte differentiation, impaired insulin action, and dysregulated adipokine secretion (274)