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For CYP3A4 inhibition testing, ketoconazole (MilliporeSigma, catalog: PHR1385) was prepared at 2 final concentration in culture medium and added in equal volume to each well of a 384-well plate for 24 h

Although the diurnal rhythm is maintained, the mean cortisol levels increase by 1.5-2 times with morning levels increasing several times higher than normal levels