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This higher frequency of sEPSC was not associated with a shift in the amplitude of the events, supporting the idea that the nAChRs responsible are expressed on presynaptic terminals, as we have reported previously (data not shown) [20] After pretreatment of slices with 1 M bupropion, nicotine still increased the spontaneous excitatory postsynaptic current (EPSC) frequency in 58% of the neurons, but the increase was reduced by 73% (Fig 2b,c)