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Only two studies (5%) were longitudinal observational studies 17,20 , while 9 (23%) were cross-sectional studies 14,16,19,21,23,24,30,32,38 , 1 (3%) was a case report 15 , and 27 (69%) were cell/ in vitro or animal studies 18,22,25-29,31,33-37,39-52

Discussion To address the gaps with prior investigations, this research developed a custom-made smoking simulation machine, specifically for dental material testing under controlled smoking conditions, that addresses critical limitations of the existing devices

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